Cores & Technologies - Cell Analysis Core

Center Cores & Technologies - High Throughput Cell Analysis Core

The High Throughput Cell Analysis Core is dedicated to the identification, functional and molecular characterization of muscle-derived cell populations of healthy and diseased adult skeletal muscle. This core includes prospective isolation of muscle-derived cell subpopulations by FACS (Muscle Sorting Service), a high throughput microscopy and screening of libraries of siRNAs, and microRNAs (miRs) and chemical compounds (High Throughput Screening Service), and in vivo cell transplantation into mouse models of disease (Cell Transplantation Service). The flow cytometry, sorting, high throughput microscopy, chemical and functional genomics screening services are available in existing cores at SBMRI but not readily accessible to the non-SBMRI muscle community.
This P30 will make these services available to the San Diego muscle research community.
cell-analysis core
The High Throughput Cell Analysis Core will interface with the Phenotyping & Physiology Core and the Imaging Core by assisting Center investigators with the appropriate isolation and characterization of muscle-derived cells from the mouse models or human tissues that are provided by the Center member. This core will make available to all Center investigators sophisticated flow-cytometry, high throughput imaging microscopy, and screening of noncoding RNAs (siRNA and miRNA) and chemical compounds libraries. Moreover, the Core will also provide assistance and training for animal irradiation and cell transplantation. Additional services include single cell analysis and transplantation from Luciferase transgenic mice, enabling the monitoring the homing, proliferation and differentiation of transplanted cells in vivo.

Core Director & Co-Directors

Director

Lorenzo Puri
Lorenzo Puri, MD, PhD


Contact:
Telephone: 858-646-3161
Fax: 858-795-5298
[email protected]

Co-Director

Alessandra Sacco
Alessandra Sacco, PhD


Contact:
Telephone: 858-795-5337
Fax: 858-713-6274
[email protected]
More information on the Core Directors and their research interests.


Specific Aims of the Core

  1. To provide training and technical assistance in FACS isolation of muscle-derived cell types from different mouse models. This will include:
    • Functional characterization in vitro of myogenic potential of sorted cell populations and evaluation of functional interaction by coculture assays.
    • Evaluation of heterogeneity of freshly isolated muscle-derived cells by clonal analyses in vitro.
  2. To provide technical assistance for high throughput microscopy and screening of non-coding RNAs (siRNA and miRNA) and chemical compounds libraries. This will include:
    • Assistance and training in assay development and screening performance of high throughput imaging microscopy and kinetic imaging microscopy.
    • Screening of collections of shRNA and miRNA libraries.
    • Screening of a large unbiased or small focused collections of chemical compounds available at the Chemical Screening Library Core at the SBMRI.
  3. To provide training and technical assistance in testing the regenerative potential of isolated muscle-derived cells by intramuscular transplantation in vivo.

Available Technologies

  • FACS analysis
  • High throughput imaging microscopy and kinetic imaging microscopy
  • Evaluation of myogenic potential in vitro and in vivo
  • Screening of shRNA, miRNA, small molecule libraries

Please contact the core directors directly with inquiries about services performed by the core facility.

Please note that the price list may be subject to change.
For latest prices, please visit the Core Webpage at Sanford-Burnham.

Price List

Service Unit External Non-Profit External For-Profit
BSL2 Sorting EA $13.50 $26.30
Countess Kit (50 slides, 100 tests) BX $141.75 $276.15
Countess Slide EA $3.38 $6.58
FACSAria Setup EA $48.60 $94.68
FACSAria Sorting HR $97.20 $189.36
FACSCanto + Staff Operator HR $81.00 $157.80
FACSCanto Independent Use HR $40.50 $78.90
FACSCanto Training HR $81.00 $157.80
FACSCanto Weekends/Evenings HR $33.75 $65.75
FACSort/FACSCalibur + Staff Operator HR $74.25 $144.65
FACSort/FACSCalibur Independent Use HR $33.75 $65.75
FACSort/FACSCalibur Training HR $74.25 $144.65
FACSort/FACSCalibur Weekends/Evenings HR $27.00 $52.60
Filter Tubes EA $2.36 $4.60
Filter Tubes (Bag of 25) BX $47.25 $92.05
Image StreamX II Assisted Use HR $108.60 $211.20
ImageStreamX II Overnight Use Each $202.50 $294.50
LSRFortessa Independent Use HR $47.25 $92.05
LSRFortessa Weekends/Evenings HR $40.50 $78.90
Plate Sorting HR $13.50 $26.30
Reference Particles EA $13.50 $26.30
Sterile Sorting EA $20.25 $39.45